Transferring:Article Title: METTL7A drives pituitary neuroendocrine tumor invasion via an m6A-dependent epigenetic mechanism upregulating EGFR/PI3K/Akt signaling
Article Snippet: .. T25 flasks (TCF012050), T75 flasks (TCF012250), 6 well-plates (TCP011006), 15 mL centrifuge tubes (CFT920150), 50 mL centrifuge tubes (CFT920500) (Jet BIOFIL, China); Non-enzymatic pipette tips (KeyGEN, China); 1.5 mL Non-enzymatic centrifuge tube (Axygen, China); PVDF membrane (IPVH00010) (Sigma-Aldrich, America) thin plate of a glass plate electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); pipettes (Eppendorf Middle East & Africa GmbH, Germany); nitrile latex gloves (Loctite); Thin plate of glass plate of electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); Transwell chamber (8 μm model: 3422) (CORNING, China); 0.2 mL PCR single tube (PCR-02C) (Axygen, China); 0.1 mL qPCR eight-strip tube (TLS0851) (BIO-RAD, USA); Fluorometric 96-well PCR plate (F603102), fluorometric nuclease-free mounting (F604419) (Sangon, China). .. Fetal bovine serum (FBS, #FSD500; Excell Bio, China); NonPreservative Penicillin-Streptomycin Solution (100x, #C0222; Beyotime, China); DMEM High Glucose, #11965118; Gibco, MA, USA); Trypsin (#R001100; Gibco, CardioGiren, China); Cell Freezing Medium (#C0210B-50 ml), PBS Buffer (#C0221A), APS (#ST005), RIPA Lysis Buffer (#P0013B), PMSF (#ST506), TEMED (#ST728; Beyotime, China); 30% Acrylamide/Bis Solution (#BL513A); Tris-Base (#BS083); TBS Buffer Powder (#BL602A); BSA Protein Standard (#BL673A); Tween-20 (#BS100; Biosharp, China); SDS (#3250); Glycine (#1275); Non-fat Milk Powder (#1172; Biolfroxx, Germany); BCA Protein Assay Kit (#WB6501), 5 × SDS-PAGE Loading Buffer (#WB2001), ECL Substrate (#P2100; NCM Biotech, Shanghai, China); methanol (#HB05; Guangzhou Chemical Reagent, China); prestained protein marker II (#G2058 - 250UL; Servicebio, China); GAPDH (14C10) rabbit mAb (#2118; Cell Signaling Technology, USA); Anti-METTL7A (ab79207, Abcam, UK), Anti-EGFR Antibody [EP38y] (ab52894, Abcam, UK), PI3K p85 alpha (U5) (Thermo Fisher, USA), Phospho-PI3K p85 alpha (Tyr607) (Thermo Fisher, USA), Akt (#9272, CST, USA), Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (#4060, CST, USA), Anti-GAPDH (6C5) (#ab8245, Abcam, UK), HRP-conjugated Goat Anti-rabbit (bs0295G-HRP, Bioss, China), HRP-Goat Anti-Mouse IgG (bs-0296G-HRP, Bioss, China); Lipofectamin 3000 (L3000015, Thermo Fisher, USA); Cell/Tissue Total RNA Extraction Kit V2 (RC112, Vazyme, China); HiScript III 1st strand cDNA Synthesis Kit (R312, Vazyme, China); Taq Pro Universal SYBR qPCR Master Mix (Q712, Vazyme, China); PCR Primer (Sangon Biotech, China); CCK-8 Cell Proliferation Assay Kit (BA00208, Bioss, China); BD Matrigel (354230, Corning, USA); 4 % Paraformaldehyde (P0099, Beyotime, China); 1 % Crystal Violet (V5265-250 ML, Sigma-Aldrich, USA); BeyoClickTM EdU-488 Cell Proliferation Kit (C0071S, Beyotime, China); RNA Immunoprecipitation (RIP) Kit (Bes5101(N), Bersin.
Membrane:Article Title: METTL7A drives pituitary neuroendocrine tumor invasion via an m6A-dependent epigenetic mechanism upregulating EGFR/PI3K/Akt signaling
Article Snippet: .. T25 flasks (TCF012050), T75 flasks (TCF012250), 6 well-plates (TCP011006), 15 mL centrifuge tubes (CFT920150), 50 mL centrifuge tubes (CFT920500) (Jet BIOFIL, China); Non-enzymatic pipette tips (KeyGEN, China); 1.5 mL Non-enzymatic centrifuge tube (Axygen, China); PVDF membrane (IPVH00010) (Sigma-Aldrich, America) thin plate of a glass plate electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); pipettes (Eppendorf Middle East & Africa GmbH, Germany); nitrile latex gloves (Loctite); Thin plate of glass plate of electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); Transwell chamber (8 μm model: 3422) (CORNING, China); 0.2 mL PCR single tube (PCR-02C) (Axygen, China); 0.1 mL qPCR eight-strip tube (TLS0851) (BIO-RAD, USA); Fluorometric 96-well PCR plate (F603102), fluorometric nuclease-free mounting (F604419) (Sangon, China). .. Fetal bovine serum (FBS, #FSD500; Excell Bio, China); NonPreservative Penicillin-Streptomycin Solution (100x, #C0222; Beyotime, China); DMEM High Glucose, #11965118; Gibco, MA, USA); Trypsin (#R001100; Gibco, CardioGiren, China); Cell Freezing Medium (#C0210B-50 ml), PBS Buffer (#C0221A), APS (#ST005), RIPA Lysis Buffer (#P0013B), PMSF (#ST506), TEMED (#ST728; Beyotime, China); 30% Acrylamide/Bis Solution (#BL513A); Tris-Base (#BS083); TBS Buffer Powder (#BL602A); BSA Protein Standard (#BL673A); Tween-20 (#BS100; Biosharp, China); SDS (#3250); Glycine (#1275); Non-fat Milk Powder (#1172; Biolfroxx, Germany); BCA Protein Assay Kit (#WB6501), 5 × SDS-PAGE Loading Buffer (#WB2001), ECL Substrate (#P2100; NCM Biotech, Shanghai, China); methanol (#HB05; Guangzhou Chemical Reagent, China); prestained protein marker II (#G2058 - 250UL; Servicebio, China); GAPDH (14C10) rabbit mAb (#2118; Cell Signaling Technology, USA); Anti-METTL7A (ab79207, Abcam, UK), Anti-EGFR Antibody [EP38y] (ab52894, Abcam, UK), PI3K p85 alpha (U5) (Thermo Fisher, USA), Phospho-PI3K p85 alpha (Tyr607) (Thermo Fisher, USA), Akt (#9272, CST, USA), Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (#4060, CST, USA), Anti-GAPDH (6C5) (#ab8245, Abcam, UK), HRP-conjugated Goat Anti-rabbit (bs0295G-HRP, Bioss, China), HRP-Goat Anti-Mouse IgG (bs-0296G-HRP, Bioss, China); Lipofectamin 3000 (L3000015, Thermo Fisher, USA); Cell/Tissue Total RNA Extraction Kit V2 (RC112, Vazyme, China); HiScript III 1st strand cDNA Synthesis Kit (R312, Vazyme, China); Taq Pro Universal SYBR qPCR Master Mix (Q712, Vazyme, China); PCR Primer (Sangon Biotech, China); CCK-8 Cell Proliferation Assay Kit (BA00208, Bioss, China); BD Matrigel (354230, Corning, USA); 4 % Paraformaldehyde (P0099, Beyotime, China); 1 % Crystal Violet (V5265-250 ML, Sigma-Aldrich, USA); BeyoClickTM EdU-488 Cell Proliferation Kit (C0071S, Beyotime, China); RNA Immunoprecipitation (RIP) Kit (Bes5101(N), Bersin.
Electrophoresis:Article Title: METTL7A drives pituitary neuroendocrine tumor invasion via an m6A-dependent epigenetic mechanism upregulating EGFR/PI3K/Akt signaling
Article Snippet: .. T25 flasks (TCF012050), T75 flasks (TCF012250), 6 well-plates (TCP011006), 15 mL centrifuge tubes (CFT920150), 50 mL centrifuge tubes (CFT920500) (Jet BIOFIL, China); Non-enzymatic pipette tips (KeyGEN, China); 1.5 mL Non-enzymatic centrifuge tube (Axygen, China); PVDF membrane (IPVH00010) (Sigma-Aldrich, America) thin plate of a glass plate electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); pipettes (Eppendorf Middle East & Africa GmbH, Germany); nitrile latex gloves (Loctite); Thin plate of glass plate of electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); Transwell chamber (8 μm model: 3422) (CORNING, China); 0.2 mL PCR single tube (PCR-02C) (Axygen, China); 0.1 mL qPCR eight-strip tube (TLS0851) (BIO-RAD, USA); Fluorometric 96-well PCR plate (F603102), fluorometric nuclease-free mounting (F604419) (Sangon, China). .. Fetal bovine serum (FBS, #FSD500; Excell Bio, China); NonPreservative Penicillin-Streptomycin Solution (100x, #C0222; Beyotime, China); DMEM High Glucose, #11965118; Gibco, MA, USA); Trypsin (#R001100; Gibco, CardioGiren, China); Cell Freezing Medium (#C0210B-50 ml), PBS Buffer (#C0221A), APS (#ST005), RIPA Lysis Buffer (#P0013B), PMSF (#ST506), TEMED (#ST728; Beyotime, China); 30% Acrylamide/Bis Solution (#BL513A); Tris-Base (#BS083); TBS Buffer Powder (#BL602A); BSA Protein Standard (#BL673A); Tween-20 (#BS100; Biosharp, China); SDS (#3250); Glycine (#1275); Non-fat Milk Powder (#1172; Biolfroxx, Germany); BCA Protein Assay Kit (#WB6501), 5 × SDS-PAGE Loading Buffer (#WB2001), ECL Substrate (#P2100; NCM Biotech, Shanghai, China); methanol (#HB05; Guangzhou Chemical Reagent, China); prestained protein marker II (#G2058 - 250UL; Servicebio, China); GAPDH (14C10) rabbit mAb (#2118; Cell Signaling Technology, USA); Anti-METTL7A (ab79207, Abcam, UK), Anti-EGFR Antibody [EP38y] (ab52894, Abcam, UK), PI3K p85 alpha (U5) (Thermo Fisher, USA), Phospho-PI3K p85 alpha (Tyr607) (Thermo Fisher, USA), Akt (#9272, CST, USA), Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (#4060, CST, USA), Anti-GAPDH (6C5) (#ab8245, Abcam, UK), HRP-conjugated Goat Anti-rabbit (bs0295G-HRP, Bioss, China), HRP-Goat Anti-Mouse IgG (bs-0296G-HRP, Bioss, China); Lipofectamin 3000 (L3000015, Thermo Fisher, USA); Cell/Tissue Total RNA Extraction Kit V2 (RC112, Vazyme, China); HiScript III 1st strand cDNA Synthesis Kit (R312, Vazyme, China); Taq Pro Universal SYBR qPCR Master Mix (Q712, Vazyme, China); PCR Primer (Sangon Biotech, China); CCK-8 Cell Proliferation Assay Kit (BA00208, Bioss, China); BD Matrigel (354230, Corning, USA); 4 % Paraformaldehyde (P0099, Beyotime, China); 1 % Crystal Violet (V5265-250 ML, Sigma-Aldrich, USA); BeyoClickTM EdU-488 Cell Proliferation Kit (C0071S, Beyotime, China); RNA Immunoprecipitation (RIP) Kit (Bes5101(N), Bersin.
Polymerase Chain Reaction:Article Title: METTL7A drives pituitary neuroendocrine tumor invasion via an m6A-dependent epigenetic mechanism upregulating EGFR/PI3K/Akt signaling
Article Snippet: .. T25 flasks (TCF012050), T75 flasks (TCF012250), 6 well-plates (TCP011006), 15 mL centrifuge tubes (CFT920150), 50 mL centrifuge tubes (CFT920500) (Jet BIOFIL, China); Non-enzymatic pipette tips (KeyGEN, China); 1.5 mL Non-enzymatic centrifuge tube (Axygen, China); PVDF membrane (IPVH00010) (Sigma-Aldrich, America) thin plate of a glass plate electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); pipettes (Eppendorf Middle East & Africa GmbH, Germany); nitrile latex gloves (Loctite); Thin plate of glass plate of electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); Transwell chamber (8 μm model: 3422) (CORNING, China); 0.2 mL PCR single tube (PCR-02C) (Axygen, China); 0.1 mL qPCR eight-strip tube (TLS0851) (BIO-RAD, USA); Fluorometric 96-well PCR plate (F603102), fluorometric nuclease-free mounting (F604419) (Sangon, China). .. Fetal bovine serum (FBS, #FSD500; Excell Bio, China); NonPreservative Penicillin-Streptomycin Solution (100x, #C0222; Beyotime, China); DMEM High Glucose, #11965118; Gibco, MA, USA); Trypsin (#R001100; Gibco, CardioGiren, China); Cell Freezing Medium (#C0210B-50 ml), PBS Buffer (#C0221A), APS (#ST005), RIPA Lysis Buffer (#P0013B), PMSF (#ST506), TEMED (#ST728; Beyotime, China); 30% Acrylamide/Bis Solution (#BL513A); Tris-Base (#BS083); TBS Buffer Powder (#BL602A); BSA Protein Standard (#BL673A); Tween-20 (#BS100; Biosharp, China); SDS (#3250); Glycine (#1275); Non-fat Milk Powder (#1172; Biolfroxx, Germany); BCA Protein Assay Kit (#WB6501), 5 × SDS-PAGE Loading Buffer (#WB2001), ECL Substrate (#P2100; NCM Biotech, Shanghai, China); methanol (#HB05; Guangzhou Chemical Reagent, China); prestained protein marker II (#G2058 - 250UL; Servicebio, China); GAPDH (14C10) rabbit mAb (#2118; Cell Signaling Technology, USA); Anti-METTL7A (ab79207, Abcam, UK), Anti-EGFR Antibody [EP38y] (ab52894, Abcam, UK), PI3K p85 alpha (U5) (Thermo Fisher, USA), Phospho-PI3K p85 alpha (Tyr607) (Thermo Fisher, USA), Akt (#9272, CST, USA), Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (#4060, CST, USA), Anti-GAPDH (6C5) (#ab8245, Abcam, UK), HRP-conjugated Goat Anti-rabbit (bs0295G-HRP, Bioss, China), HRP-Goat Anti-Mouse IgG (bs-0296G-HRP, Bioss, China); Lipofectamin 3000 (L3000015, Thermo Fisher, USA); Cell/Tissue Total RNA Extraction Kit V2 (RC112, Vazyme, China); HiScript III 1st strand cDNA Synthesis Kit (R312, Vazyme, China); Taq Pro Universal SYBR qPCR Master Mix (Q712, Vazyme, China); PCR Primer (Sangon Biotech, China); CCK-8 Cell Proliferation Assay Kit (BA00208, Bioss, China); BD Matrigel (354230, Corning, USA); 4 % Paraformaldehyde (P0099, Beyotime, China); 1 % Crystal Violet (V5265-250 ML, Sigma-Aldrich, USA); BeyoClickTM EdU-488 Cell Proliferation Kit (C0071S, Beyotime, China); RNA Immunoprecipitation (RIP) Kit (Bes5101(N), Bersin.
Real-time Polymerase Chain Reaction:Article Title: METTL7A drives pituitary neuroendocrine tumor invasion via an m6A-dependent epigenetic mechanism upregulating EGFR/PI3K/Akt signaling
Article Snippet: .. T25 flasks (TCF012050), T75 flasks (TCF012250), 6 well-plates (TCP011006), 15 mL centrifuge tubes (CFT920150), 50 mL centrifuge tubes (CFT920500) (Jet BIOFIL, China); Non-enzymatic pipette tips (KeyGEN, China); 1.5 mL Non-enzymatic centrifuge tube (Axygen, China); PVDF membrane (IPVH00010) (Sigma-Aldrich, America) thin plate of a glass plate electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); pipettes (Eppendorf Middle East & Africa GmbH, Germany); nitrile latex gloves (Loctite); Thin plate of glass plate of electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); Transwell chamber (8 μm model: 3422) (CORNING, China); 0.2 mL PCR single tube (PCR-02C) (Axygen, China); 0.1 mL qPCR eight-strip tube (TLS0851) (BIO-RAD, USA); Fluorometric 96-well PCR plate (F603102), fluorometric nuclease-free mounting (F604419) (Sangon, China). .. Fetal bovine serum (FBS, #FSD500; Excell Bio, China); NonPreservative Penicillin-Streptomycin Solution (100x, #C0222; Beyotime, China); DMEM High Glucose, #11965118; Gibco, MA, USA); Trypsin (#R001100; Gibco, CardioGiren, China); Cell Freezing Medium (#C0210B-50 ml), PBS Buffer (#C0221A), APS (#ST005), RIPA Lysis Buffer (#P0013B), PMSF (#ST506), TEMED (#ST728; Beyotime, China); 30% Acrylamide/Bis Solution (#BL513A); Tris-Base (#BS083); TBS Buffer Powder (#BL602A); BSA Protein Standard (#BL673A); Tween-20 (#BS100; Biosharp, China); SDS (#3250); Glycine (#1275); Non-fat Milk Powder (#1172; Biolfroxx, Germany); BCA Protein Assay Kit (#WB6501), 5 × SDS-PAGE Loading Buffer (#WB2001), ECL Substrate (#P2100; NCM Biotech, Shanghai, China); methanol (#HB05; Guangzhou Chemical Reagent, China); prestained protein marker II (#G2058 - 250UL; Servicebio, China); GAPDH (14C10) rabbit mAb (#2118; Cell Signaling Technology, USA); Anti-METTL7A (ab79207, Abcam, UK), Anti-EGFR Antibody [EP38y] (ab52894, Abcam, UK), PI3K p85 alpha (U5) (Thermo Fisher, USA), Phospho-PI3K p85 alpha (Tyr607) (Thermo Fisher, USA), Akt (#9272, CST, USA), Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (#4060, CST, USA), Anti-GAPDH (6C5) (#ab8245, Abcam, UK), HRP-conjugated Goat Anti-rabbit (bs0295G-HRP, Bioss, China), HRP-Goat Anti-Mouse IgG (bs-0296G-HRP, Bioss, China); Lipofectamin 3000 (L3000015, Thermo Fisher, USA); Cell/Tissue Total RNA Extraction Kit V2 (RC112, Vazyme, China); HiScript III 1st strand cDNA Synthesis Kit (R312, Vazyme, China); Taq Pro Universal SYBR qPCR Master Mix (Q712, Vazyme, China); PCR Primer (Sangon Biotech, China); CCK-8 Cell Proliferation Assay Kit (BA00208, Bioss, China); BD Matrigel (354230, Corning, USA); 4 % Paraformaldehyde (P0099, Beyotime, China); 1 % Crystal Violet (V5265-250 ML, Sigma-Aldrich, USA); BeyoClickTM EdU-488 Cell Proliferation Kit (C0071S, Beyotime, China); RNA Immunoprecipitation (RIP) Kit (Bes5101(N), Bersin.
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